The Homocysteine (Hcy) assay kit provides an convenient and accurate measurement of Hcy concentration in human serum. Test principleThe plasma Hcy concentrations are measured by enzyme conversion method. Hcy is first reduced by the oxidant and is decomposed by the gene recombinase afterwards. The product react with the chromogenic agent, emitting fluorescent light of 710 nm while being simulated by the light of 660 nm. The plasma Hcy concentrations can be calculated from the fluorescence differentials. Product Features
1. Linear range of Hcy level: [1, 100] μmol/L Linear correlation coefficient ∣r∣≥0.990 Linear deviation ≤ ± 10.00%
2. AccuracyRelative deviation is within ±10%.
3. PrecisionIntra CV% ≤ 5.0%; inter CV% ≤ 5.0%.Technical Parameters
Reference range of Hcy level: 5 - 15 μmol/L.
|Temperature||37ºC||Optical path length (OPL)||1.0 cm|
|Exicitation wavelength||660 nm||Emission wavelength||710 nm|
|Reaction time||10 min|| || |
Packing SpecificationsR1a: 1×12.5 ml, R1b: 2×30 cuvettes, R2: 1×2 ml (for 60 tests)Storage and stabilityUnopened: Store test kits at 2 - 8ºC protected from light. Stable to expiration date printed on individual vials (for 16 months).Opened: Store test kits at 2 - 8ºC protected from light. Stable for 1 month if kept capped from original container and free from contamination.Do not freeze the test kit.